Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. Unlike competitor mixes that can exhibit inhibition at high cdna inputs and low correlation coefficients over a dynamic range experiment. 1 generating consistent amplification across a wide dynamic range is fundamental to qpcr methodology and use of the δδct method for gene expression analysis.
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