If you have not yet used them we recommend trying our new ssoadvanced universal sybr green and ssoadvanced universal probes supermixes as superior performance alternatives for use on any real time pcr instrument. Quantitative rt pcr protocol sybr green i 5 3 running 3 agarose gel after qrt pcr 4 analyze qrt pcr data. In rna quantitation assays you use the sybr green pcr master mix in the second step of a two step reverse transcription polymerase chain reaction rt pcr protocol.
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